【dmem培养基】DMEM培养基培养什么细胞可以培养F81嘛

网友回答

【答案】 可以养很多细胞的,DMEM（意思就是双倍（double） 盐的 MEM）比MEM营养更丰富,DMEM又分为好多种的.
　　常用细胞所用培养基配方
　　CELL LINE CELL TYPE SPECIES TISSUE MEDIUM
　　293 fibroblast human embryonic kidney MEM,10% HI horse serum
　　3T6 fibroblast mouse embryo DMEM,10% FBS
　　A549 epithelial human lung carcinoma F-12K,10% FBS
　　A9 fibroblast mouse connective tissue DMEM,10% FBS
　　AtT-20 epithelial mouse pituitary tumor F-10,15% horse serum and 2.5% FBS
　　BALB/3T3 fibroblast mouse embryo DMEM,10% FBS
　　BHK-21 fibroblast hamster kidney GMEM,10% FBS or MEM,10% FBS and NEAA
　　BHL-100 epitheliall human breast McCoy'5A,10% FBS
　　BT fibroblast bovine turbinate cells MEM,10% FBS and NEAA
　　Caco-2 epithelial human colon adeno carcinoma MEM,20% FBS and NEAA
　　Chang epithelial human liver BME,10% calf serum
　　CHO-K1 epithelial hamster ovary F-12,10% FBS
　　Clone 9 epithelial rat liver F-12K,10% FBS
　　Clone M-3 epithelial mouse melanoma F-10,15% horse serum and 2.5% FBS
　　COS-1 fibroblast monkey kidney DMEM,10% FBS
　　COS-3 fibroblast monkey kidney DMEM,10% FBS
　　COS-7 fibroblast monkey kidney DMEM,10% FBS
　　CRFK epithelial cat kidney MEM,10% FBS and NEAA
　　CV-1 fibroblast monkey kidney MEM,10% FBS
　　D-17 epithelial dog osteosara MEM,10% FBS and NEAA
　　Daudi lymphoblast human blood from a lymphoma patient RPMI-1640,10% FBS
　　GH1 epithelial rat pituitary tumor F-10,15% horse serum and 2.5% FBS
　　GH3 epithelial rat pituitary tumor F-10,15% horse serum and 2.5% FBS
　　H9 lymphoblast human T-cell lymphoma RPMI-1640,20% FBS
　　HaK epithelial hamster kidney BME,10% calf serum
　　HCT-15 epithelial human colorectal adenocarcinoma RPMI-1640,10% FBS
　　HeLa epithelial human cervix carcinoma MEM,10% FBS and NEAA (in suspension,S-MEM)
　　HEp-2 epithelial human larynx carcinoma MEM,10% FBS
　　HL-60 lymphoblast human promyeolocytic leukemia RPMI-1640,20% FBS
　　HT-1080 epithelial human fibrosara MEM,10% HI FBS and NEAA
　　HT-29 epithelial human colon adenocarcinoma McCoy's 5A,10% FBS
　　HUVEC endothelial human umbilical cord F-12K,10% FBS and heparinat 100 ug ml
　　I-10 epithelial mouse testicular tumor F-10,15% horse serum and 2.5% FBS
　　IM-9 lymphoblast human marrow from myeloma patient RPMI-1640,10% FBS
　　JEG-2 epithelial human choriocarcinoma MEM,10% FBS
　　Jensen fibroblast rat sara McCoy's 5A,5% FBS
　　Jurkat lyphoblast human lymphoma RPMI-1640,10% FBS
　　K-562 lymphoblast human myelogenous leukemia RPMI-1640,10% FBS
　　KB epithelial human oral carcinoma MEM,10% FBS and NEAA
　　KG-1 myeloblast human marrow from erythroleukemia patient IMDM,20% FBS
　　L2 epithelial rat lung F-12K,10%FBS
　　L6 rat skeletal muscle myoblasts DMEM,10% FBS
　　LLC-WRC 256 epithelial rat carcinoma Medium 199,5% horse serum
　　McCoy fibroblast mouse unknown MEM,10% FBS
　　MCF7 epithelial human breast adenocarcinoma MEM,10% FBS NEAA,10ug/ml insulin
　　WEHI-3b macrophage-like mouse myelomonocyte leukemia DMEM,10% FBS
　　WI-38 epithelial human embryonic lung BME,10% FBS
　　WISH epithelial human amnion BME,10% FBS
　　WS1 human embryonic skin MEM,10% FBS and NEAA
　　XC epithelial rat sara MEM,10% FBS and NEAA
　　Y-1 epithelial mouse tumor of adrenal F-10,15% horse serum and 2.5% FBS