【薄层层析】为什么薄层层析时层析缸要盖住盖子?

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【答案】 The chamber needs to be saturated with the eluent vapor.If the top is left off some of the vapor evaporates.When this happens the Rf values will tend to be lower than expected due to the evaporation.
　　The TLC needs to be in a closed container to ensure maximun resolution between ponents.